Highly specific label-free molecular imaging with spectrally tailored excitation-stimulated Raman scattering (STE-SRS) microscopy : Nature Photonics : Nature Publishing Group
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AbstractIntroductionPrincipleResultsDiscussionMethodsReferencesAcknowledgements Author informationSupplementary information ● Email ● Download PDF ● Download citation ● Order reprints ● Rights and permissions ● Share/bookmark Label-free microscopy that has chemical contrast and high acquisition speeds up to video rates has recently been made possible using stimulated Raman scattering (SRS) microscopy. SRS imaging offers high sensitivity, but the spectral specificity of the original narrowband implementation is limited, making it difficult to distinguish chemical species with overlapping Raman bands. Here, we present a highly specific imaging method that allows mapping of a particular chemical species in the presence of interfering species, based on tailored multiplex excitation of its vibrational spectrum. This is implemented by spectral modulation of a broadband pump beam at a high frequency (>1 MHz), allowing detection of the SRS signal of the narrowband Stokes beam with high sensitivity. Using the scheme, we demonstrate quantification of cholesterol in the presence of lipids, and real-time three-dimensional spectral imaging of protein, stearic acid and oleic acid in live Caenorhabditis elegans. Figures at a glance left 1. Figure 1: Principle of SRS. Articles 1. Nonlinear silicon photonics Nature Photonics 30 Jul 2010 2. Coherent coupling between distant excitons revealed by two-dimensional nonlinear hyperspectral imaging Nature Photonics 19 Dec 2010 3. Raman injection laser Nature 24 Feb 2005 4. Ultralong quantum optical data storage using an optical locking technique Nature Photonics 23 Aug 2009 5. Dicke quantum phase transition with a superfluid gas in an optical cavity Nature 29 Apr 2010 View all Open innovation challenges ● Low-Volume Liquid Dispersion Mechanism Deadline: Feb 2
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Highly specific label-free molecular imaging with spectrally tailored excitation stimulated Raman scattering (STE-SRS) microscopy.
Label-free microscopy with chemical contrast and high acquisition speed up to video-rate has recently been made possible by stimulated Raman scattering (SRS) microscopy. While SRS imaging offers superb sensitivity, the spectral specificity of the original narrowband implementation is limited, making distinguishing chemical species with overlapping Raman bands difficult. Here we present a highly...
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